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The working process and advantages of a fully automated ELISA instrument
2024-12-09
An automated microplate reader quantitatively or qualitatively analyzes substances in a sample by measuring the chemical signals produced during an enzymatic reaction. Its core principle is to add enzyme labels and substrates to the sample; the enzymatic reaction will produce measurable optical signals (such as absorbance or fluorescence). The microplate reader reads the intensity of the optical signals produced by the reaction to determine the concentration or content of the sample.
Specifically, the working process of a microplate reader is as follows:
1. Sample loading: First, add the sample to be tested to the microplate (usually a 96-well plate), with each well corresponding to a sample or sample group.
2. Addition of reaction substrate: Add specific enzyme labels and substrates to each well. These substrates will undergo a chemical reaction under the action of the enzyme, producing signals that can be detected optically.
3. Reaction process: After the enzyme reacts with the substrate, a certain amount of product will be generated. The rate and amount of the product are proportional to the concentration of the substance to be measured in the sample.
4. Data acquisition: The automated microplate reader uses its built-in optical system to detect the light absorbance (OD value) or fluorescence value of each well and converts the results into data.
5. Data analysis: Quantitative results are obtained by analyzing the data using computer software.
Automated microplate readers have many significant advantages over traditional manual microplate readers:
1. High-throughput detection: Automated microplate readers can process multiple samples simultaneously, greatly improving experimental efficiency. Modern automated microplate readers typically have detection capabilities of 96 wells, 384 wells, or even more, meeting the needs of high-throughput screening.
2. Automated operation: Unlike manual operation, automated microplate readers can automatically complete multiple tasks such as sample loading, reaction, washing, and detection, reducing errors and tedious processes in manual operation and ensuring the consistency and stability of the experiment.
3. High-precision measurement: Because automated microplate readers use advanced optical detection technology, they can provide high-precision measurement results, with accuracy reaching the nanometer level, suitable for research and clinical environments with strict requirements for detection results.
4. Saves time and manpower: Through automated processes, automated microplate readers significantly save the time and manpower required for experiments, reducing the burden on laboratory staff and improving laboratory efficiency.
5. Multifunctionality: Many automated microplate readers have multiple detection modes, such as absorbance detection, fluorescence detection, and chemiluminescence detection, to meet different experimental needs. They can not only perform traditional ELISA detection but also be used for other types of biochemical detection, such as **detection**, microbial detection, etc.
6. Intelligent data analysis: Modern automated microplate readers are usually equipped with powerful software systems that can automatically perform data analysis and report generation. Laboratory personnel only need to focus on the results, greatly improving the efficiency and accuracy of data processing.
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